ABSTRACT
AIM:To investigate the effect of microRNA(miR)-451 by targeting proteasome subunit βtype 8 (Psmb8)on the inflammatory responses in mouse glomerular mesangial cells(MCs)under high-and low-glucose condi-tions.METHODS:The expression levels of miR-451,IL-18 mRNA and TNF-αmRNA were detected by qPCR.The pro-tein expression levels of IL-18,TNF-αand Psmb8 were determined by Western blot when miR-451 was over-expressed and down-expressed in the MCs.Moreover,the expression of IL-18 and TNF-αwas detected when Psmb8 was silenced by si-Psmb8 in MCs.RESULTS:The expression of miR-451 was significantly decreased in the MCs treated with high glucose compared with low glucose group(P<0.01).However,the expression of Psmb8 was increased in high glucose group as compared with low glucose group(P<0.01).Moreover, the expression levels of Psmb8, IL-18 and TNF-αwere signifi-cantly decreased when miR-451 was over-expressed in high glucose group(P<0.01).Additionally,the expression levels of IL-18 and TNF-αwere significantly reduced when Psmb8 was silenced in the MCs under high glucose condition.CON-CLUSION:miR-451 reduces the expression of inflammatory factors via targeting Psmb8 in the MCs under high glucose condition.Therefore,miR-451 may play a role in inflammation of diabetic nephropathy.
ABSTRACT
AIM: To explore the curative effect of methylprednisolone with octreotide retrobulbar injection therapy on thyroid associated ophthalmopathy. METHODS:Fifty- one cases ( 96 eyes ) with thyroid associated ophthalmopathy were treated with methylprednisolone ( 500mg/d ) for 3d, and then retrobulbar injection of octreotide (0. 1mg/mo) for 3mo in one course. Symptoms and signs of the patients were observed before and after the treatment course. RESULTS: Symptoms of 49 cases were improved significantly. In all cases, the mean value of exophthalmus of the patients was significantly decreased after the treatment by 3. 1 ± 1. 4mm. The height of palpebral fissure was significantly decreased after the treatment by 2. 4 ± 0. 9mm, there were statistically significant difference before and after treatment (PCOCLUSION: Methylprednisolone with octreotide retrobulbar injection therapy can improve symptoms and signs of the patients with thyroid associated ophthalmopathy with high safety and no obvious side effect.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the value of transarterial chemoembolization (TACE) using mixed emboli for hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>188 patients with HCC were divided into two groups according to the treatment modality: 103 patients in group A treated by routine iodine embolus agent; 85 patients in group B by mixed iodine embolus agent (ultra-liquified iodinized oil + gelatin sponge + chemotherapeutic agents). The pattern of the arrested iodine deposition in the tumor, response, resectability during follow-up, pathological changes, survival and complications in the two groups were analyzed and compared.</p><p><b>RESULTS</b>The pattern of full-and-dense iodine deposition in the tumor and the response rate (CR + PR) were 59.2% and 32.0% in group A, 89.4% and 56.5% in group B. Surgical resection after TACE was possible in 5.8% (6/103) of group A versus 15.3% (13/85) of group B. Complete tumor necrosis was observed in 1.0% and 4.7% in groups A and B, respectively. 1-, 2- and 3-year actual survival rates were 57.7%, 42.8% and 8.4% in group A, and 79.8%, 55.3%, 38.5% in group B. The difference in results between the two groups was statistically significant, however, the incidence of complication in the two groups was similar.</p><p><b>CONCLUSION</b>Transarterial chemoembolization with mixed iodine emboli is more effective than with the routine iodine emboli in the treatment of bulky or nodular hepatocellular carcinoma rich in blood supply. Mixed iodine emboli is tolerable without increase in severe complications.</p>